hCMEC/D3 cell viability assessed by MTT assay (a) and cytotoxicity assessed by LDH assay (b) when exposed for 4 h to non-functionalized SLN RSV (), functionalized SLN-DSPE-ApoE RSV (), functionalized SLN-Palmitate-ApoE RSV () and free resveratrol () at different concentrations of resveratrol (from 10 to 100 μM). All values represent the mean standard deviation (n = 3). Results were analyzed and compared with EBM-2 medium which represents the maximum of cell viability and the minimum of cytotoxicity. * denotes statistically significant differences (P
a Permeability profile and b P app of free resveratrol (), non-functionalized SLNs RSV (), SLN-Palmitate-ApoE RSV () and SLN-DSPE-ApoE RSV () over 4 h of transport across hCMEC/D3 cell monolayer mimicking BBB permeability conditions. All values represent the mean standard deviation (n = 3). Results of resveratrol-loaded SLNs were analyzed and compared to free-resveratrol. * denotes statistically significant differences (P
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